Part:BBa_J34100:Design

AND gate, 2x PoPS input, 1x PoPS output

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 15
  Illegal EcoRI site found at 2963
  Illegal XbaI site found at 27
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 15
  Illegal EcoRI site found at 2963
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 15
  Illegal EcoRI site found at 2963
  Illegal BamHI site found at 9
  Illegal XhoI site found at 3048
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 15
  Illegal EcoRI site found at 2963
  Illegal XbaI site found at 27
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 15
  Illegal EcoRI site found at 2963
  Illegal XbaI site found at 27
  Illegal NgoMIV site found at 1968
  Illegal NgoMIV site found at 2087
  Illegal NgoMIV site found at 2702
  Illegal NgoMIV site found at 2711
  Illegal AgeI site found at 2262
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The initial promoters are omitted, so that the light sensing system can accept PoPs from another device, and the promoter on the end produces PoPs from this device

Source

When both the mRNA for the t7RNA polymerase and the tRNA (loading glutamate on the stop codon TTT) that deactivates the early stop transcription are present, a functional t7RNA polymerase can be produced.

References

Simon L. Dove and Ann Hochschild, Conversion of the v subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target, Genes and development 12:745–754

James C. Hu, Michael G. Kornacker, and Ann Hochschild, Escherichia coli One- and Two-Hybrid Systems for the Analysis and Identification of Protein Protein Interactions, Methods 20, 80-94 (2000)